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Multidrug resistance-associated protein 2 (MRP2) in plasma membrane vesicles from human erythrocytes and fibroblasts was found to be expressed in human monocytic THP-1 cells. The relative specific activity of MRP2 was higher in THP-1 cells as compared to the parental line, U937, and to THP-1 variant U937 V3 cells with a cloned multidrug resistance phenotype. THP-1 cells showed a high resistance to vincristine, daunorubicin, mitoxantrone and estramustine, which was partly blocked by verapamil. The intracellular accumulation of calcein was moderately increased in THP-1 cells, whereas the uptake of propidium iodide (PI) was unaffected. In contrast, the activity of membrane-associated gamma-glutamyl transferase was increased in THP-1 cells. The susceptibility of THP-1 cells to cisplatin was high. Our data indicate that MRP2 confers a low level of resistance in human monocytic THP-1 cells.Heat Sign Vinny Del Negro As Assistant Coach
Posted by Staff sports writer on Aug 17, 2015
The Chicago Bulls announced today that they have signed Vinny Del Negro to be an assistant coach on Head Coach Fred Hoiberg’s staff. Del Negro, who led the Golden State Warriors to consecutive Western Conference Finals appearances and the 2015 NBA title, replaces Luke Walton in the Bulls’ coaching staff.
Del Negro served as the Warriors Head Coach for the last two seasons. During his tenure with the team, he helped guide the Warriors to a 40-26 record and a berth in the Western Conference Finals in 2014-15. Under his leadership the Warriors won 34 of their last 45 regular season games. He also managed to bring
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