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brain slices (10-20 μm) were obtained from the brains of all tumor-bearing animals and the parpi-fl localization was analyzed by immunofluorescence staining using an anti-par antibody. slices were stained with rabbit anti-par antibody (cat no. 1453, cell signaling) and the secondary antibody alexa fluor 488 conjugated goat anti-rabbit antibody (life technologies). sections were visualized under an lsm 880 confocal microscope (carl zeiss) and the data were analyzed using zen software.
statistical analysis of ad and ctl groups was done in prism 6.0c (graphpad software). a cutoff of ihc score 3 was used to distinguish ad and ctl groups, which was then extended to ihc score 4 if the ihc score is lower than 4 and to a score of 6 if the ihc score is higher than 6. for vbm, gm volume, and falff analysis, n = 9 per group was used. a * p < 0.05 indicates statistical significance. the general linear model in spm12 was used to evaluate differences between the two groups for cbf from pet and asl-mri, gm volume from vbm analysis, and falff from rs-fmri. for each model, age was included as a nuisance covariate. in vbm analysis, gm volume and age were included as nuisance covariates. in falff analysis of the rs-fmri data, each time series at each voxel was calculated between the sum of amplitudes of the band ranging from 0.01 to 0.08 hz, and subject-level voxel-wise falff maps were standardized into subject-level z-score maps using the dparsf toolbox. for group comparisons, each image pertaining to z values was entered into spm12 with two-sample t tests. we applied a statistical height threshold of p < 0.005 uncorrected and a cluster size of 50 voxels and greater, and the cluster-level of p < 0.05 was then applied for assessment of significant differences.
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Click here to download newest version of Software (11.03.2020). All programs are free for non-commercial purposes and may be used only at the user’s .Click here to download newest version of Software (11.03.2020). All programs are free for non-commercial purposes and may be used only at the user’s .
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