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The effects of amino-acids, glutamine and glutathione on the behaviour and biochemistry of copper-intoxicated rats.
Rats maintained on a copper-deficient diet develop early in their life-time an impaired growth, hypochloraemia, elevated serum transaminase activities and increased plasma copper concentrations. The animals are susceptible to subcutaneous doses of copper acetate. Rats fed a low copper diet, deprived of essential amino-acids and with low levels of glutathione in the body, become more sensitive to copper. The inhibition of growth is manifested only after significant tissue depletion of copper and glutathione. The copper-intoxicated animals, even when maintained on a copper-deficient diet and deprived of essential amino-acids and glutathione, have a significantly lower mortality rate than the copper-intoxicated controls. Studies of excreted bile acids, and of the activities of alkaline phosphatase, aldolase and superoxide dismutase in bile and serum, indicate no adverse effect of amino-acids on the metabolism of copper-intoxicated animals. The copper-intoxicated glutathione-depleted rats are more sensitive to hypoxia and acidosis than the copper-intoxicated controls. The findings suggest that glutathione, and perhaps glutamine, are not essential in the treatment of copper poisoning.Pre-weaning diet transition and the development of novel markers of intestinal dysfunction in juvenile
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