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An evaluation was made of the distribution and morphology of opioid receptors, and of the innervation and microvascular supply of the lateral crest of the dorsal bursa (LCDB) in the horse. Morphological distribution of opioid receptors was determined by receptor autoradiography using [3H]-diprenorphine as a tritiated ligand, and by immunohistochemistry using a polyclonal antibody to Leucine-Enkephalin (Leu-ENK). A few dorsal horn motoneurons were found to be immunoreactive for Leu-ENK. Using [3H]-diprenorphine, receptor sites were located on the bursal wall, in the bursal tissue and in the bursal fat and these three components could be discriminated from each other by density. The predominant opioid receptor subtype, mu, was located on the bursal wall and bursal fat and could be distinguished from other receptor subtypes by differential radioligand binding. Distribution of Leu-ENK differed from the distribution of mu receptors and the presence of Leu-ENK indicated that an opioid peptide(s) of intrinsic origin was present in the LCDC. A retrograde axonal tracer was used in combination with a lectin marker to determine whether dorsal horn neurons synapse on the LCDC. Nerve endings positive for both lectin and tracer were located within the LCDC in the dorsal horn of spinal cord, and within the marginal zone of the lumbosacral dorsal root. The microvascular supply of the LCDC appeared to be predominantly provided by branches of the superficial epigastric and profunda femoris arteries.Robotic Thyroidectomy With Nerve Paravascularization Technique for Differentiated Thyroid Cancer: Short-Term Results From the FIRST and SECOND Studies.
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